Ascellularpowerplants,mitochondriaarethesubcellularorganellesfoundinmosteukaryoticcells.Theycontaintheenzymesfortricarboxylicacidcycleandproteincomplexesforelectrontransportchain.Mitochondrialdysfunctionhasbeenimplicatedinmanyhumandiseasesrelatingtocancer,diabetes,cardiacfailuresandneurodegenerativedisorders.Therefore,thereisanincreasingneedforaneffectivemethodtoisolateintactandfunctionalmitochondriafromfreshorfrozentissuesandculturedcells.Sciencell"sMitochondriaIsolationKithasbeendesignedtoprovidetheoptimalyieldofintactandenzymaticallyactivemitochondria.
Thebasicprinciplesofmitochondriaisolationusingthiskitincludethreestages:
1)mechanicalrupturingoftissuesorcells
2)removingcellulardebrisandnucleibylowspeedcentrifugationand
3)harvestingmitochondriabyhighspeedcentrifugation.
Specifications:
CatalogNo. | 8268 |
CountryofManufacture | UnitedStates |
ProductCode | MITOISO |
Size/Quantity | 100reactions |
ProductUse | Thisproductisforresearchpurposesonlyandnotforuseinanimals,humans,ordiagnosticprocedures. |
Storage | Storein-20°C |
ShippingInfo | Dryice. |
References | TrounceIA,KimYL,JunAS,WallaceDC.1996.Assessmentofmitochondrialoxidativephosphorylationinpatientmuscle.biopsies,lymphoblasts,andtransmitochondrialcelllines.MethodsEnzymol264:484�509. |