Sciencell"sGeneQuery™HumanCDNAEvaluationKit(GQH-CE)assessescDNAquality.ThekitverifiessuccessfulreversetranscriptionofmessengerRNA(mRNA)tocomplementaryDNA(cDNA)andrevealsthepresenceofgenomicDNA(gDNA)contaminationincDNAsamples.GoodqualitycDNAisacriticalcomponentforsuccessfulgeneexpressionprofiling.TheGQH-CEkitishighlyrecommendedforcDNAapplicationssuchasGeneQuery™qPCRarrays.
EachprimersetincludedinGQH-CEqPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters:(i)anoptimalannealingtemperatureof65°C(with2mMMg2+,andnoDMSO);(ii)recognitionofallknowntargetgenetranscriptvariants;and(iii)specificamplificationofonlyoneamplicon.EachprimersethasbeenvalidatedbyqPCRbymeltcurveanalysisandgelelectrophoresis.
QualityControl
EachprimersetisvalidatedbyqPCRmeltcurveandamplificationcurveanalyses.ThePCRproductsareanalyzedbygelelectrophoresistoconfirmsinglebandamplification.
Specifications:
CatalogNo. | GK992 |
CountryofManufacture | UnitedStates |
ProductCode | GQH-CE |
Size/Quantity | One96-wellplate,one20ulreactionperwell |
ProductUse | GQH-CEisforresearchuseonly.Itisnotapprovedforhumanoranimaluseorforapplicationinclinicalorinvitrodiagnosticprocedures. |
Storage | Thisproductisshippedatambienttemperature.Uponreceipt,thevialsshouldbestoredat4°Candaregoodforupto12months.Forlong-termstorage(>1year),storethevialsat-20°Cinamanualdefrostfreezer. |
ShippingInfo | Ambienttemperature. |