Sciencell"sGeneQuery™HumanCDNAEvaluationKit,Deluxe(GQH-CED)assessescDNAquality.ThekitverifiessuccessfulreversetranscriptionofmessengerRNA(mRNA)tocomplementaryDNA(cDNA),revealsthepresenceofgenomicDNA(gDNA)contaminationincDNAsamples,anddetectsqPCRinhibitorcontamination.GoodqualitycDNAisacriticalcomponentforsuccessfulgeneexpressionprofiling.TheGQH-CEDkitishighlyrecommendedforcDNAapplicationssuchasGeneQuery™qPCRarrays.
EachprimersetincludedinGQH-CEDqPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters:(i)anoptimalannealingtemperatureof65°C(with2mMMg2+,andnoDMSO);(ii)recognitionofallknowntargetgenetranscriptvariants;and(iii)specificamplificationofonlyoneamplicon.EachprimersethasbeenvalidatedbyqPCRbymeltcurveanalysisandgelelectrophoresis.
QualityControl
EachprimersetisvalidatedbyqPCRmeltcurveandamplificationcurveanalyses.ThePCRproductsareanalyzedbygelelectrophoresistoconfirmsinglebandamplification.
Specifications:
CatalogNo. | GK991 |
CountryofManufacture | UnitedStates |
ProductCode | GQH-CED |
Size/Quantity | 100Reactions |
ProductUse | GQH-CEDisforresearchuseonly.Itisnotapprovedforhumanoranimaluseorforapplicationinclinicalorinvitrodiagnosticprocedures. |
Storage | Thisproductisshippedatambienttemperature.Uponreceipt,thevialsshouldbestoredat4°Candaregoodforupto12months.Forlong-termstorage(>1year),storethevialsat-20°Cinamanualdefrostfreezer. |
ShippingInfo | Ambienttemperature. |