ThiskitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"sCDNA.
Note:allgeneandpseudogenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
PseudogenesareDNAsegmentsintroducedintothegenomebygeneduplicationorRNAretrotransposition.Themajorityofpseudogenesbearhighsequencesimilaritytotheircorrespondingfunctionalgenes,buthavelostsomeorallofthefunctionality.Sciencell"sGeneQuery™HumanVIM-VIM2PPseudogeneTranscriptionAnalysisqPCRKit(GQP-VIM2P)isdesignedtofacilitatethetranscriptquantificationofpseudogeneVIM2Panditshighlyrelatedgene,VIM.HumanVIM2PpseudogeneandVIMgenearelocatedonchromosome6and10,respectively. GQP-VIM2Pguaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutnon-specificamplificationforprecisequantification.
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"scDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
Specifications:
| CatalogNo. | GK814 | 
| CountryofManufacture | UnitedStates | 
| ProductCode | GQP-VIM2P | 
| Size/Quantity | |
| ProductUse | |
| Storage | |
| ShippingInfo | |

		