
ThiskitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"sCDNA.
Note:allgeneandpseudogenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
PseudogenesareDNAsegmentsintroducedintothegenomebygeneduplicationorRNAretrotransposition.Themajorityofpseudogenesbearhighsequencesimilaritytotheircorrespondingfunctionalgenes,buthavelostsomeorallofthefunctionality.Sciencell"sGeneQuery™HumanTMPO-TMPOP2PseudogeneTranscriptionAnalysisqPCRKit(GQP-TMPOP2)isdesignedtofacilitatethetranscriptquantificationofpseudogeneTMPOP2anditscorrespondingfunctionalgene,TMPO.HumanTMPOP2pseudogeneandTMPOgenearelocatedonchromosome16and12,respectively. AlthoughTMPOgeneandTMPOP2pseudogenesharehighsequencesimilarity,GQP-TMPOP2guaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutcross-amplificationforprecisequantification.
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"scDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
Specifications:
CatalogNo. | GK813 |
CountryofManufacture | UnitedStates |
ProductCode | GQP-TMPOP2 |
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