PseudogenesareDNAsegmentsintroducedintothegenomebygeneduplicationorRNAretrotransposition.Themajorityofpseudogenesbearhighsequencesimilaritytotheircorrespondingfunctionalgenes,buthavelostsomeorallofthefunctionality.Sciencell"sGeneQuery™HumanSUMO1-SUMO1P3PseudogeneTranscriptionAnalysisqPCRKit(GQP-SUMO1P3)isdesignedtofacilitatethetranscriptquantificationofpseudogeneSUMO1P3anditscorrespondingfunctionalgene,SUMO1.HumanSUMO1P3pseudogeneandSUMO1genearelocatedonchromosome1and2,respectively. AlthoughSUMO1geneandSUMO1P3pseudogenesharehighsequencesimilarity,GQP-SUMO1P3guaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutcross-amplificationforprecisequantification.
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"scDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
Specifications:
CatalogNo. | GK812 |
CountryofManufacture | UnitedStates |
ProductCode | GQH-SUMO1P3 |
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