ThiskitsareqPCRreadyina96-wellplateformat, witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"sCDNA.
Note:allgeneandpseudogenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
PseudogenesareDNAsegmentsintroducedintothegenomebygeneduplicationorRNAretrotransposition.Themajorityofpseudogenesbearhighsequencesimilaritytotheircorrespondingfunctionalgenes,buthavelostsomeorallofthefunctionality.Sciencell"sGeneQuery™HumanSMUG1-SMUG1P1PseudogeneTranscriptionAnalysisqPCRKit(GQP-SMUG1P1)isdesignedtofacilitatethetranscriptquantificationofpseudogeneSMUG1P1anditscorrespondingfunctionalgene,SMUG1.HumanSMUG1P1pseudogeneandSMUG1genearelocatedonchromosome18and12,respectively. AlthoughSMUG1geneandSMUG1P1pseudogenesharehighsequencesimilarity,GQP-SMUG1P1guaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutcross-amplificationforprecisequantification.
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"scDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
Specifications:
| CatalogNo. | GK811 |
| CountryofManufacture | UnitedStates |
| ProductCode | GQP-SMUG1P1 |
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