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Sciencell/GeneQuery™ Human MYLK-MYLKP1 Pseudogene Transcription Analysis qPCR Kit/GK810/1 Ea
ThiskitsareqPCRreadyina96-wellplateformat, witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"sCDNA.
Note:allgeneandpseudogenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
PseudogenesareDNAsegmentsintroducedintothegenomebygeneduplicationorRNAretrotransposition.Themajorityofpseudogenesbearhighsequencesimilaritytotheircorrespondingfunctionalgenes,buthavelostsomeorallofthefunctionality.Sciencell"sGeneQuery™HumanMYLK-MYLKP1PseudogeneTranscriptionAnalysisqPCRKit(GQP-MYLKP1)isdesignedtofacilitatethetranscriptquantificationofpseudogeneMYLKP1anditscorrespondingfunctionalgene,MYLK.HumanMYLKP1pseudogeneandMYLKgenearebothlocatedonchromosome3. AlthoughMYLKgeneandMYLKP1pseudogenesharehighsequencesimilarity,GQP-MYLKP1guaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutcross-amplificationforprecisequantification.
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"scDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
Specifications:
CatalogNo. | GK810 |
CountryofManufacture | UnitedStates |
ProductCode | GQP-MYLKP1 |
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