ThiskitsareqPCRreadyina96-wellplateformat, witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"sCDNA.
Note:allgeneandpseudogenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
PseudogenesareDNAsegmentsintroducedintothegenomebygeneduplicationorRNAretrotransposition.Themajorityofpseudogenesbearhighsequencesimilaritytotheircorrespondingfunctionalgenes,buthavelostsomeorallofthefunctionality.Sciencell"sGeneQuery™HumanMT1A-MT1DPPseudogeneTranscriptionAnalysisqPCRKit(GQP-MT1DP)isdesignedtofacilitatethetranscriptquantificationofpseudogeneMT1DPanditshighlyrelatedgene,MT1A.HumanMT1DPpseudogeneandMT1Agenearebothlocatedonchromosome16.GQP-MT1DPguaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutnon-specificamplificationforprecisequantification.
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"scDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
Specifications:
| CatalogNo. | GK809 | 
| CountryofManufacture | UnitedStates | 
| ProductCode | GQP-MT1DP | 
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