ThiskitsareqPCRreadyina96-wellplateformat, witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"sCDNA.
Note:allgeneandpseudogenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
PseudogenesareDNAsegmentsintroducedintothegenomebygeneduplicationorRNAretrotransposition.Themajorityofpseudogenesbearhighsequencesimilaritytotheircorrespondingfunctionalgenes,buthavelostsomeorallofthefunctionality.Sciencell"sGeneQuery™HumanKRAS-KRASP1PseudogeneTranscriptionAnalysisqPCRKit(GQP-KRASP1)isdesignedtofacilitatethetranscriptquantificationofpseudogeneKRASP1anditscorrespondingfunctionalgene,KRAS.HumanKRASP1pseudogeneandKRASgenearelocatedonchromosome6and12,respectively. AlthoughKRASgeneandKRASP1pseudogenesharehighsequencesimilarity,GQP-KRASP1guaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutcross-amplificationforprecisequantification.
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"scDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
Specifications:
| CatalogNo. | GK808 |
| CountryofManufacture | UnitedStates |
| ProductCode | GQP-KRASP1 |
| Size/Quantity | |
| ProductUse | |
| Storage | |
| ShippingInfo | |

