
ThiskitsareqPCRreadyina96-wellplateformat, witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"sCDNA.
Note:allgeneandpseudogenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
PseudogenesareDNAsegmentsintroducedintothegenomebygeneduplicationorRNAretrotransposition.Themajorityofpseudogenesbearhighsequencesimilaritytotheircorrespondingfunctionalgenes,buthavelostsomeorallofthefunctionality.Sciencell"sGeneQuery™HumanOTOF-FER1L4PseudogeneTranscriptionAnalysisqPCRKit(GQP-FER1L4-B)isdesignedtofacilitatethetranscriptquantificationofpseudogeneFER1L4anditshighlyrelatedgene,OTOF.HumanFER1L4pseudogeneandOTOFgenearelocatedonchromosome20and2,respectively.GQP-FER1L4-Bguaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutnon-specificamplificationforprecisequantification.
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"scDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
Specifications:
CatalogNo. | GK807 |
CountryofManufacture | UnitedStates |
ProductCode | GQP-FER1L4-B |
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