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Sciencell/GeneQuery™ 人ARHGAP27-ARHGAP27P1假基因转录分析qPCR试剂盒/GK805/1 Ea

ThiskitsareqPCRreadyina96-wellplateformat, witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"sCDNA.
Note:allgeneandpseudogenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
PseudogenesareDNAsegmentsintroducedintothegenomebygeneduplicationorRNAretrotransposition.Themajorityofpseudogenesbearhighsequencesimilaritytotheircorrespondingfunctionalgenes,buthavelostsomeorallofthefunctionality.Sciencell"sGeneQuery™HumanARHGAP27-ARHGAP27P1PseudogeneTranscriptionAnalysisqPCRKit(GQP-ARHGAP27P1)isdesignedtofacilitatethetranscriptquantificationofpseudogeneARHGAP27P1anditscorrespondingfunctionalgene,ARHGAP27.HumanARHGAP27P1pseudogeneandARHGAP27genearebothlocatedonchromosome17. AlthoughARHGAP27geneandARHGAP27P1pseudogenesharehighsequencesimilarity,GQP-ARHGAP27P1guaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutcross-amplificationforprecisequantification.
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"scDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
Specifications:
CatalogNo. | GK805 |
CountryofManufacture | UnitedStates |
ProductCode | GQP-ARHGAP27P1 |
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