ThiskitsareqPCRreadyina96-wellplateformat, witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"sCDNA.
Note:allgeneandpseudogenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
PseudogenesareDNAsegmentsintroducedintothegenomebygeneduplicationorRNAretrotransposition.Themajorityofpseudogenesbearhighsequencesimilaritytotheircorrespondingfunctionalgenes,buthavelostsomeorallofthefunctionality.Sciencell"sGeneQuery™HumanZNF300-ZNF300P1PseudogeneTranscriptionAnalysisqPCRKit(GQP-ZNF300P1)isdesignedtofacilitatethetranscriptquantificationofpseudogeneZNF300P1anditscorrespondingfunctionalgene,ZNF300.HumanZNF300P1pseudogeneandZNF300genearebothlocatedonchromosome5. AlthoughZNF300geneandZNF300P1pseudogenesharehighsequencesimilarity,GQP-ZNF300P1guaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutcross-amplificationforprecisequantification.
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"scDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
Specifications:
CatalogNo. | GK804 |
CountryofManufacture | UnitedStates |
ProductCode | GQP-AOC4P |
Size/Quantity | One96-wellplate,one20ulreactionperwell |
ProductUse | Forresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationinclinicalorinvitrodiagnosticprocedures. |
Storage | Storeat4°Cuponreceipt.Forlong-termstorage(>1year),storetheplateat-20°Cinamanualdefrostfreezer. |
ShippingInfo | Ambienttemperature. |