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Sciencell/GeneQuery™ Human LILRB3-LILRP2 Pseudogene Transcription Analysis qPCR Kit/GK802/1 Ea
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"sCDNA.
Note:allgeneandpseudogenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
PseudogenesareDNAsegmentsintroducedintothegenomebygeneduplicationorRNAretrotransposition.Themajorityofpseudogenesbearhighsequencesimilaritytotheircorrespondingfunctionalgenes,buthavelostsomeorallofthefunctionality.Sciencell"sGeneQuery™HumanLILRB3-LILRP2PseudogeneTranscriptionAnalysisqPCRKit(GQP-LILRP2)isdesignedtofacilitatethetranscriptquantificationofpseudogeneLILRP2/CD85M,anditshighlyrelatedgene,LILRB3/CD85A.HumanLILRB3geneandLILRP2pseudogenearebothlocatedonchromosome19. AlthoughLILRB3geneandLILRP2pseudogenesharehighsequencesimilarity,GQP-LILRP2guaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutcross-amplificationforprecisequantification.
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"scDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
Specifications:
CatalogNo. | GK802 |
CountryofManufacture | UnitedStates |
ProductCode | GQP-LILRP2 |
Size/Quantity | One96-wellplate,one20ulreactionperwell |
ProductUse | Forresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationinclinicalorinvitrodiagnosticprocedures. |
Storage | Storeat4°Cuponreceipt.Forlong-termstorage(>1year),storetheplateat-20°Cinamanualdefrostfreezer. |
ShippingInfo | Ambienttemperature. |