Kit(GQP-PTENP1)isdesignedtofacilitatethetranscriptquantificationofpseudogenePTENP1anditscorrespondingfunctionalgene,PTEN.
Note:allgeneandpseudogenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
Sciencell"sGeneQuery™HumanPTEN-PTENP1PseudogeneTranscriptionAnalysisqPCRKit(GQP-PTENP1)isdesignedtofacilitatethetranscriptquantificationofpseudogenePTENP1anditscorrespondingfunctionalgene,PTEN.HumanPTENP1pseudogeneandPTENgenearelocatedonchromosome9and10,respectively. AlthoughPTENgeneandPTENP1pseudogenesharehighsequencesimilarity,GQP-PTENP1guaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutcross-amplificationforprecisequantification.
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"sCDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
Specifications:
CatalogNo. | GK801 |
CountryofManufacture | UnitedStates |
ProductCode | GQP-PTENP1 |
Size/Quantity | One96-wellplate,one20ulreactionperwell |
ProductUse | Forresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationinclinicalorinvitrodiagnosticprocedures. |
Storage | Storeat4°Cuponreceipt.Forlong-termstorage(>1year),storetheplateat-20°Cinamanualdefrostfreezer. |
ShippingInfo | Ambienttemperature. |