EachprimersetincludedinHMSC-C-qPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters:(i)anoptimalannealingtemperatureof65°C(with2mMMg2+,andnoDMSO);(ii)recognitionofallknowntargetgenetranscriptvariants;and(iii)specificamplificationofonlyoneamplicon.
Humanmesenchymalstemcells(MSCs)areapopulationofmultipotentcellsthatcanbedifferentiatedintomultiplelineage-specificcells,whichcanformbone,fat,cartilage,muscleandtendon.Theprocessofcartilageformationfromcondensedmesenchymetissueisknownaschondrogenesis.SciencellhascreatedaconvenientqPCRkitfortheassessmentofhumanmesenchymalstemcellchondrogenesis.SOX9andCOL2A1qPCRprimersincludedinthekitallowforthedetectionandquantificationofearly-to-mid-andlate-stageofhumanMSCchondrogenesis,respectively1.
Note:allgenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
FourqPCRcontrolsareincludedinthiskittoverifysuccessfulreversetranscriptionofmessengerRNA(mRNA)tocomplementaryDNA(CDNA),revealthepresenceofgenomicDNA(gDNA)contaminationincDNAsamples,anddetectqPCRinhibitorcontamination.GoodqualitycDNAisacriticalcomponentforsuccessfulgeneexpressionanalysis.
EachprimersetincludedinHMSC-C-qPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters:(i)anoptimalannealingtemperatureof65°C(with2mMMg2+,andnoDMSO);(ii)recognitionofallknowntargetgenetranscriptvariants;and(iii)specificamplificationofonlyoneamplicon.EachprimersethasbeenvalidatedbyqPCRbymeltcurveanalysisandgelelectrophoresis.
Specifications:
| CatalogNo. | 8328 | 
| CountryofManufacture | UnitedStates | 
| ProductCode | HMSC-C-qPCR | 
| Size/Quantity | 50reactions | 
| ProductUse | HMSC-CPCRkitisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures. | 
| Storage | Storeat-20°Cuponreceipt.Avoidrepeatedfreezethawcyclesbymakingsixaliquotsat110μLeach. | 
| ShippingInfo | Dryice. | 
| References | [1]Sekiya,I.,Vuoristo,J.T.,Larson,B.L.,&Prockop,D.J.(2002).Invitrocartilageformationbyhumanadultstemcellsfrombonemarrowstromadefinesthesequenceofcellularandmoleculareventsduringchondrogenesis.PNAS,99(7),4397-4402. | 

		