Mesenchymalstemcells(MSC)areawell-characterizedpopulationofadultstemcells.Theyhavethepotentialtodevelopintomaturecellsthatproducefat,cartilage,bone,tendons,andmuscle[1,2].Theseproperties,incombinationwiththeirdevelopmentalplasticity,havegeneratedtremendousinterestbecauseofthepotentialuseofMSCinregenerativemedicine.MSCculturedwithoutseruminthepresenceoftransformationgrowthfactorswilldifferentiateintochondrocytes.Incontrast,MSCculturedinserumwithascorbicacidanddexamethasonewilldifferentiateintoosteoblasts.MSChavethecapABIlityforrenewalanddifferentiationintovariouslineagesofmesenchymaltissues.Withtheirrenewalcapability,MSChavethepotentialtobetransplantedintoaninjuredsiteorseededonabiomimeticscaffoldtogenerateappropriatetissueconstructs.
HVMSCfromSciencellResearchLaboratoriesareisolatedfromhumanvertebralbody.HVMSCarecryopreservedatpassageoneanddeliveredfrozen.Eachvialcontains>5x10^5cellsin1mlvolume.HVMSCarecharacterizedbyimmunofluorescencewithantibodiesspecifictoCD73,CD90,CD105andlipidstainingafterdifferentiation.HVMSCarenegativeforHIV-1,HBV,HCV,mycoplasma,bacteria,yeastandfungi.HVMSCareguaranteedtofurtherexpandfor15populationdoublingsundertheconditionsprovidedbyScienCellResearchLaboratories.
RecommendedMedium
ItisrecommendedtouseMesenchymalStemCellMedium(MSCM,Cat.#7501)forculturingHVMSCinvitro.
Specifications:
CatalogNo. | 7550 |
CountryofManufacture | UnitedStates |
ProductCode | HVMSC |
Size/Quantity | 5x10^5cells/vial |
ProductUse | HVMSCareforresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
Storage | Directlyandimmediatelytransfercellsfromdryicetoliquidnitrogenuponreceivingandkeepthecellsinliquidnitrogenuntilcellcultureisneededforexperiments. |
ShippingInfo | Dryice. |
References | 1.Kassem,M.Mesenchymalstemcells:BIOLOGicalcharacteristicsandpotentialclinicalapplications.2004.CloningStemCells.6(4):369-74.2.Barry,F.P.,andJ.M.Murphy.Mesenchymalstemcells:clinicalapplicationsandbiologicalcharacterization.2004.IntJBiochemCellBiol.36(4):568-84. |