Mesenchymalstemcells(MSC)areawell-characterizedpopulationofadultstemcells.Theyhavethepotentialtodevelopintomaturecellsthatproducefat,cartilage,bone,tendons,andmuscle[1,2].Theseproperties,incombinationwiththeirdevelopmentalplasticity,havegeneratedtremendousinterestbecauseofthepotentialuseofMSCinregenerativemedicine.Thefetalliveristhesourceofearlyhematopoieticcellsandisconsideredasourceofpluripotentstemcells[3].MSCculturedwithoutseruminthepresenceoftransformationgrowthfactorswilldifferentiateintochondrocytes.Incontrast,MSCculturedinserumwithascorbicacidanddexamethasonewilldifferentiateintoosteoblasts.MSChavethecapABIlityforrenewalanddifferentiationintovariouslineagesofmesenchymaltissues.Withtheirrenewalcapability,MSChavethepotentialtobetransplantedintoaninjuredsiteorseededonabiomimeticscaffoldtogenerateappropriatetissueconstructs.
HMSC-hefromSciencellResearchLaboratoriesareisolatedfromhumanliver.HMSC-hearecryopreservedatpassageoneanddeliveredfrozen.Eachvialcontains>5x10^5cellsin1mlvolume.HMSC-hearecharacterizedbyimmunofluorescencewithantibodiesspecifictoCD73,CD90,CD105andlipidstainingafterdifferentiation.HMSC-hearenegativeforHIV-1,HBV,HCV,mycoplasma,bacteria,yeastandfungi.HMSC-heareguaranteedtofurtherexpandfor15populationdoublingsundertheconditionsprovidedbyScienCellResearchLaboratories.
RecommendedMedium
ItisrecommendedtouseMesenchymalStemCellMedium(MSCM,Cat.#7501)forculturingHMSC-heinvitro.
Specifications:
CatalogNo. | 7520 |
CountryofManufacture | UnitedStates |
ProductCode | HMSC-he |
Size/Quantity | 5x10^5cells/vial |
ProductUse | HMSC-heareforresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
Storage | Directlyandimmediatelytransfercellsfromdryicetoliquidnitrogenuponreceivingandkeepthecellsinliquidnitrogenuntilcellcultureisneededforexperiments. |
ShippingInfo | Dryice. |
References | [1]Kassem,M.(2004)Mesenchymalstemcells:BIOLOGicalcharacteristicsandpotentialclinicalapplications.CloningStemCells.6(4):369-74.[2]Barry,F.P.,andJ.M.Murphy.(2004)Mesenchymalstemcells:clinicalapplicationsandbiologicalcharacterization.IntJBiochemCellBiol.36(4):568-84.[3]Machaj,EK,Grabowska,I,Gajkowska,A,Jastrzewska,M,Oldak,T,Moraczewski,JandPojda,Z.(2005)Differentiationpotentialofthefetalratliver-derivedcells.FoliaHistochemCytobiol.43(4):217-22. |