Mesenchymalstemcells(MSC)areawell-characterizedpopulationofadultstemcells.Theyhavethepotentialtodevelopintomaturecellsthatproducefat,cartilage,bone,tendons,andmuscle[1,2].Theseproperties,incombinationwiththeirdevelopmentalplasticity,havegeneratedtremendousinterestbecauseofthepotentialuseofMSCinregenerativemedicinetoreplacedamagedtissues.MSCculturedwithoutseruminthepresenceoftransformationgrowthfactorswilldifferentiateintochondrocytes.Incontrast,MSCculturedinserumwithascorbicacidanddexamethasonewilldifferentiateintoosteoblasts.MSChavethecapABIlityforrenewalanddifferentiationintovariouslineagesofmesenchymaltissues.Withtheirrenewalcapability,MSChavethepotentialtobetransplantedintoaninjuredsiteorseededonabiomimeticscaffoldtogenerateappropriatetissueconstructs.
HMSC-adfromSciencellResearchLaboratoriesareisolatedfromhumanADIposetissue.HMSC-adarecryopreservedatpassageoneanddeliveredfrozen.Eachvialcontains>5x10^5cellsin1mlvolume.HMSC-adarecharacterizedbyimmunofluorescencewithantibodiesspecifictoCD73,CD90,CD105andlipidstainingafterdifferentiation.HMSC-adarenegativeforHIV-1,HBV,HCV,mycoplasma,bacteria,yeastandfungi.HMSC-adareguaranteedtofurtherexpandfor15populationdoublingsundertheconditionsprovidedbyScienCellResearchLaboratories.
RecommendedMedium
ItisrecommendedtouseMesenchymalStemCellMedium(MSCM,Cat.#7501)forculturingHMSC-adinvitro.
Specifications:
CatalogNo. | 7510 |
CountryofManufacture | UnitedStates |
ProductCode | HMSC-ad |
Size/Quantity | 5x10^5cells/vial |
ProductUse | HMSC-adareforresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
Storage | Directlyandimmediatelytransfercellsfromdryicetoliquidnitrogenuponreceivingandkeepthecellsinliquidnitrogenuntilcellcultureisneededforexperiments. |
ShippingInfo | Dryice. |
References | 1.Kassem,M.Mesenchymalstemcells:BIOLOGicalcharacteristicsandpotentialclinicalapplications.2004.CloningStemCells.6(4):369-74.2.Barry,F.P.,andJ.M.Murphy.Mesenchymalstemcells:clinicalapplicationsandbiologicalcharacterization.2004.IntJBiochemCellBiol.36(4):568-84. |