Isolatedfromembryonicday13CD1mouseembryos.Thesecellsarecryopreservedatprimarycultureanddeliveredfrozen.Eachvialcontains1x10^6cellsin1mlvolume.

MouseEmbryonicFibroblasts(MEF)areusedtosupportthegrowthofmouseandhumanpluripotentstemcells[1].MEFnotonlyprovideasubstrateforpluripotentstemcellstogrowon,butalsosecretecriticalgrowthfactorstomaintainstemcellpluripotency.MEFareisolatedfrommouseembryosandusedatearlypassages[2].Toserveasfeedercells,MEFmustbetreatedwithmitomycinCorbyirrADIationtopreventcellproliferation.Thetreatedcellscanalsobeusedtogenerateconditionedmediumforfeeder-freecultureofpluripotentstemcells.

MEFfromSciencellResearchLaboratoriesareisolatedfromembryonicday13CD1mouseembryos.Thesecellsarecryopreservedatprimarycultureanddeliveredfrozen.Eachvialcontains1x10^6cellsin1mlvolume.MEFarecharacterizedbyimmunofluorescencewithantibodyspecifictofibronectin.MEFarenegativeformycoplasma,bacteria,yeast,andfungi.MEFareguaranteedtofurtherexpandfor5populationdoublingsundertheconditionsprovidedbyScienCellResearchLaboratories.

RecommendedMedium

ItisrecommendedtouseDMEM(Cat.#09221)supplementedwith10%fetalbovineserum(FBS,Cat.#0010,0025,0500)forculturingMEFinvitro.

Specifications:

CatalogNo.	M7540
CountryofManufacture
ProductCode	MEF
Size/Quantity	1x10^6cells/vial
ProductUse	MEFareusedasafeederlayerinmouseandhumanpluripotentstemcellculture.Theyareforresearchuseonly.Theyarenotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures..
Storage	Uponreceiving,directlyandimmediatelytransferthecellsfromdryicetoliquidnitrogenandkeepthecellsinliquidnitrogenuntiltheyareneededforexperiments.
ShippingInfo	Dryice.
References	[1]BRADLEY,A.(1987).Productionandanalysisofchimaeras.InTeratocarcinomasandEmbryonicStemCells:APracticalApproach,(ed.E.J.Robertson),pp.113-151.Oxford:IRLPress.[2]Nagyetal.(2006)PreparingMouseEmbryoFibroblastsColdSpringHarborProtocols.2006:pdb.prot4398.

ScienCellScienCell研究实验室是一家成立于1999年的不断扩大的生物技术公司。主要为研究界提供各种高质量的正常人和动物细胞，细胞培养基和试剂，基因分析工具，细胞衍生的分子生物学产品，基于细胞的检测试剂盒和干细胞产品。ScienCell Research Laboratories is an expanding biotechnology company established in 1999. Our mission is to research and develop cell products for experimental and therapeutic use.ScienCell provides a variety of high quality normal human and animal cells, cell culture media and reagents, gene analysis tools, cell-derived molecular biology products, cell-based assay kits, and stem cell products for the research community.To complement our vast array of primary human and animal cells, we offer specialty medium designed to selectively promote unique cell growth, including STEMium™, which allows for the optimal growth of human pluripotent stem cells under feeder-free conditions. The Scientists at ScienCell are studying and developing cell therapeutic strategies to significantly improve the quality of life for patients with degenerative diseases.产品列表：No.品牌货号名称规格1sciencell7652MAMMARY EPITHELIAL CELL GROWTH SUPPLEMENT5ml2sciencell7611MAMMARY EPITHELIAL CELL MEDIUM500ml3sciencell1001Endothelial Cell Medium500ml4sciencell1001-01Recombinant Protein A5mg5sciencell1052Endothelial Cell Growth Supplement5ml6sciencell0113Trypsin Neutralization Solution100 ml7sciencell0103Trypsin/EDTA Solution, 0.25%100ml8sciencell0123Enzyme-free Cell Dissociation Solution100ml9sciencell0133Cell Freezing Medium50ml10sciencell0143Cell Freezing Medium-serum free50ml11sciencell0153Human Pluripotent Stem Cell Cryopreservation Medium50ml12sciencell0163Human Pluripotent Stem Cell Cryopreservation Medium 5*10ml13sciencell0183Trypsin/EDTA Solution, 0.05%100ml14sciencell0203Trypan Blue Solution (0.4%)50ml15sciencell0223Alizarin Red S Staining Kit100ml