Sciencell™BovinePlasmaVitronectinispurifiedfrombovineplasmabyaffinitychromatography.ItissuppliedasasterilesolutioninDulbecco"sPhosphate-BufferedSaline(DPBS).
Caution:Ifhandledimproperly,somecomponentsofthisproductmaypresentahealthhazard.Takeappropriateprecautionswhenhandlingthisproduct,includingthewearingofprotectiveclothingandeyewear.Disposeofproperly.
Vitronectinisaglycoproteinfoundinplasmaandextracellularmatrix(ECM).Itisasoluble,disulfide-linkeddimer,composedofa75kDaanda65kDapeptidechain.Inplasma,vitronectinispredominantlyfoundasasinglechainmonomer[1].TheN-terminalofvitronectincontainsmultiplebindingsitesforavarietyofstructures.VitronectinisinvolvedinanumberofBIOLOGicalfunctionsincludingcelladhesion,spreADIng,migration,proliferation,extracellularanchoring,fibrinolysis,hemostasis,andcomplementimmunedefense[2,3,4].Vitronectincanbeusedforcoatingtissueculturesurfacestopromotecelladhesion,proliferation,anddifferentiation.
ScienCell™BovinePlasmaVitronectinispurifiedfrombovineplasmabyaffinitychromatography[5].ItissuppliedasasterilesolutioninDulbecco"sPhosphate-BufferedSaline(DPBS).Optimalconditionsforcellattachmentmustbedeterminedforeachcelllineandapplication.
ProductSpecification
Quantity: 100µg
Concentration: 0.4mg/ml
Storagebuffer: DPBS,pH7.4
Specifications:
CatalogNo. | 8538 |
CountryofManufacture | UnitedStates |
ProductCode | BPV |
Size/Quantity | 100μg |
ProductUse | THESEPRODUCTSAREFORRESEARCHUSEONLY.Notapprovedforhumanorveterinaryuse,forapplicationtohumansoranimals,orforuseinclinicalorinvitroprocedures. |
Storage | Itisrecommendedthattheproductbealiquotedandstoredat-80°C.Vitronectinshouldbethawedslowlyat2-8°Cwithnoagitation.Anyprecipitatethatispresentcanberemovedbycentrifugation.Avoidrepeatedfreeze/thawcycles. |
ShippingInfo | Dryice. |
References | 1.VuentoM,KorkolainenM,KuuselaP,HolttaE.(1985)"Isolationofanovelcell-attachmentandspreading-promotingproteinfromhumanserum."BiochemJ.227:421-7.2.HaymanEG,PierschbacherMD,SuzukiS,RuoslahtiE.(1985)"Vitronectin--amajorcellattachmentpromotingproteininfetalbovineserum."ExpCellRes.160:245-58.3.AkiyamaKS.(1999)"PurificationofVitronectin."CurrentProtocolsinCellBiology.10.6.1-10.6.5 |