1vialoffrozenHPSC-CC(Cat.#6240,1mL,>1.5x10^6cells/vial)
50mLCardiomyocytePlatingMedium-basal(Cat.#5921)
1mL50xCardiomyocytePlatingMediumSupplement(Cat.#5972)
250mLCardiomyocyteGrowthMedium-basal(Cat.#5901D)
5mLCardiomyocyteGrowthMediumSupplement(Cat.#5952)
Thisitemwillbeavailabletoshipapproximately3weeksaftertheorderissubmitted
Humanprimarycardiomyocytesandcardiactissuearesuperiormodelingsystemsforheartdiseasestudies,drugdiscoveryandtoxicitytestingcomparedtoanimalcellsorimmortalizedcelllines.HumanadultprimarycardiomyocytesaredifficulttoobtainduetolimitedavailABIlityofcardiactissueandtheydonottypicallyproliferateinculture.Thecardiomyocytesderivedfromhumanpluripotentstemcells(hPSC)canpotentiallyprovideanunlimitedsupplyofcardiomyocytesfordrugscreening,toxicitytestinganddevelopmentalstudies.
HPSC-derivedCardiomyocyteCells(HPSC-CC)ofSciencellResearchLaboratoriesaredifferentiatedfromhPSCandenrichedforcontractingcells.ThroughthetemporalmodulationofcanonicalWntsignalingandgrowthfactorinduction,monolayer-culturedhPSCaredifferentiatedtocontractingcardiomyocytesunderserum-freeandfeeder-freeconditionsathighefficiency.HPSC-CCarecryopreservedatpassageoneafterenrichmentanddeliveredfrozen. Eachvialcontains>1.5x10^6 cellsin1mlvolume.Cellsarecharacterizedbyimmunofluorescencewithantibodiesspecifictoalpha-actininandsarcomerictropomyosin.Afterreviving,HPSC-CCwillbegincontractingwithin48hourswhenculturedinCardiomyocyteGrowthMedium(CGM,Cat.#5901D).HPSC-CCarenegativeforHIV-1,HBV,HCV,mycoplasma,bacteria,yeastandfungi. HPSC-CCareguaranteedtofurthercultureundertheconditionsprovidedbyScienCellResearchLaboratories.HPSC-CCarenotrecommendedforexpandingsincethecellsshowlimitedproliferationcapabilityinCardiomyocyteGrowthMedium(CGM,Cat.#5901D)inculture.
BeatingcardiomyocytesderivedfromHDFiPSCs
Specifications:
| CatalogNo. | 6240 |
| CountryofManufacture | UnitedStates |
| ProductCode | HPSC-CC |
| Size/Quantity | 1.5x10^6cells/vial |
| ProductUse | HPSC-CCareforresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
| Storage | Uponreceiving,directlyandimmediatelytransferthecellsfromdryicetoliquidnitrogenandkeepthecellsinliquidnitrogenuntiltheyareneededforexperiments. |
| ShippingInfo | Dryice. |
