ECM-NGconsistsof500mlofbasalmedium,25mloffetalbovineserum(FBS,Cat.#0025),5mlofEndothelialCellGrowthSupplement(ECGS,Cat.#1052and5mlofpenicillin/streptomycinsolution(P/S,Cat.#0503).Note:FBS,ECGSandP/Sarenotpre-mixedinECM-NG;theymustbeaddedseparatelytomakethecompleteECM-NG.
Thisitemwillbeavailabletoshipapproximately3weeksaftertheorderissubmitted
Endothelialcellmedium–noglutamine(ECM-NG),whenusedwithEndothelialCellGrowthSupplement(ECGS)andFBSisacompletemediumdesignedforoptimalgrowthofnormalendothelialcellsinvitro.Itisasterile,liquidmediumwhichcontainsessentialandnon-essentialaminoacids,vitamins,organicandinorganiccompounds,hormones,growthfactors,tracemineralsandalowconcentrationoffetalbovineserum(5%).ThemediumisHEPESandbicarbonatebufferedandhasapHof7.4whenequilibratedinanincubatorwithanatmosphereof5%CO2/95%air.Themediumisformulated(quantitativelyandqualitatively)toprovideanoptimallybalancednutritionalenvironmentthatselectivelysupportsthegrowthofnormalendothelialcellsinvitro.
ECM-NGdoesnotcontainL-glutamine,anessentialaminoacidthatisunstableinsolutionat37°C.ECM-NGshouldbesupplementedwithL-glutamine(L-Glu;cat#0813)orstableL-glutaminedipeptide,L-alanyl-l-glutamine(LALG,Cat.#0733).TherecommendedLALGconcentrationrangesfrom2mMto10mMbutmayvarywithcultureconditions.
Specifications:
CatalogNo. | 1001-NG |
CountryofManufacture | UnitedStates |
ProductCode | ECM-NG |
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