Thespleenstoresandpurifieserythrocytes,metabolizeshemoglobin,andrecyclesiron.Thespleenalsoprovidesacriticalfunctionfortheimmunesystembymountingaprimaryimmuneresponsetoantigensinthebloodandsynthesizingantibodies[1].SplenocytesaremononuclearcellsderivedfromthespleenandincludeT-lymphocytes,B-lymphocytes,NK-cells,andNKTcells.Primarymousesplenocytes(MS)canbeusedtoisolateCD4+T-cells,CD8+T-cellsandCD45R+Bcells.
MSfromScienCellResearchLaboratoriesareisolatedfromnormaladultC57BL/6mousespleen.MSaredepletedofsplenicmacrophages,cryopreservedafterisolation,anddeliveredfrozen.Eachvialcontainsatleast10millioncellsin1mlvolume.MSarequalitycontroltestedforviABIlity.MSarenegativeformycoplasma,bacteria,yeast,andfungi.MScanbemaintainedforalimitedperiodoftimeincultureusingtheconditionsprovidedbyScienCellResearchLaboratoriesandarenotintendedforlong-termculture.
Specifications:
CatalogNo. | M5540-57 |
CountryofManufacture | UnitedStates |
ProductCode | MS |
Size/Quantity | 10millioncellsin1mlvolume |
ProductUse | MSareforresearchuseonly.Theyarenotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
Storage | Uponreceiving,directlyandimmediatelytransferthecellsfromdryicetoliquidnitrogenandkeepthecellsinliquidnitrogenuntiltheyareneededforexperiments. |
ShippingInfo | Dryice. |
References | [1]MebiusR,KraalG.(2005)"Structureandfunctionofthespleen."NatRevImmunol5(8):606-616 |