Macrophagesarecellsdifferentiatedfromcirculatingbonemarrow-derivedmonocytes.Inthebonemarrowandsubsequestlyinthebloodandtissues,monocytesundergoacascadeofmaturationthatculminateinmorphologicallyandfunctionallymaturetissuemacrophages[1].MacrophagesfunctiontoremovalcellulardebrisandinvADIngpathogens.Theyphagocytizeinvadingmicroorganisms,andscavengedead,damagedcellsandcellulardebris.MacrophagescanbeidentifiedbythespecificexpressionofseveralcellsurfaceproteinsincludingCD14,CD11b,F4/80(mice)/EMR1(human),MAC-1/MAC-3,andCD68byflowcytometryorimmunohistochemicalstaining[2].
MMa-bmfromSciencellResearchLaboratoriesareisolatedfromadultmousebonemarrow.MMa-bmareharvestedatP0anddeliveredfrozen.Eachvialcontains>1x10^6cellsin1mlvolume.MMa-bmarecharacterizedbyimmunofluorescencewithantibodyspecifictoCD11bandF4/80. MMa-bmarenegativeformycoplasma,bacteria,yeast,andfungi.MMa-bmareguaranteedtofurthercultureundertheconditionsprovidedbyScienCellResearchLaboratories;however,MMa-bmarenotrecommendedforexpandingorlong-termculturessincethecellsdonotproliferateinregularculture.
RecommendedMedium
ItisrecommendedtouseMacrophageMedium(MaM,Cat.#1921)fortheculturingofMMa-bminvitro.
Specifications:
CatalogNo. | M1920-57 |
CountryofManufacture | UnitedStates |
ProductCode | MMa-bm |
Size/Quantity | 1x10^6cells/vial |
ProductUse | MMa-bmareforresearchuseonly.Theyarenotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
Storage | Transfercellsdirectlyandimmediatelyfromdryicetoliquidnitrogenuponreceivingandkeepthecellsinliquidnitrogenuntilcellcultureisneededforexperiments. |
ShippingInfo | Dryice. |
References | [1].ClineMJandSumnerMA.(1972)BoneMarrowMacrophagePrecursors.I.SomeFunctionalCharacteristicsoftheEarlyCellsoftheMouseMacrophageSeries.Blood.40:62-69.[2].WynnTAandBarronL.(2010)Macrophages:masterregulatorsofinflammationandfibrosis.SeminLiverDis.30:245-57.[3].GordonSandTaylorRP.(2005)Monocyteandmacrophageheterogeneity.NatureReviewsImmunology.5:953-964. |