MHMafromScienCellResearchLaboratoriesareisolatedfrompostnatalday2mouseliver.MHMaarecryopreservedafterpurificationanddeliveredfrozen.Eachvialcontains>1x10^6cellsin1mlvolume.
Macrophagesarecellsdifferentiatedfromcirculatingbonemarrow-derivedmonocytes.ThemainfunctionofmacrophagesistoremovecellulardebrisanddestroyinvADIngpathogens.MouseHepaticMacrophages(MHMa),whicharealsoknownasKupffercells,residewithinthelumenofliversinusoids.MHMaprotecttheliverbyrespondingtopathogensandmetastaticcells,whiletoleratingharmlessselfandforeignantigens,whichenterviabloodflowthroughtheportalveinandhepaticartery[1].Recentstudieshaveshownthathepaticmacrophagesplayanimportantroleinfibrosis,liverinflammation,fattyliverdisease,andlivertransplantation[2-4].MHMaareanexcellentmodelforstudyingmacrophagefunctionsundernormalphysiologicalandpathologicalconditions.
MHMafromScienCellResearchLaboratoriesareisolatedfrompostnatalday2mouseliver.MHMaarecryopreservedafterpurificationanddeliveredfrozen.Eachvialcontains>1x10^6cellsin1mlvolume.MHMaarecharacterizedbyimmunofluorescencewithantibodytoF4/80.MHMaarenegativeformycoplasma,bacteria,yeast,andfungi.MHMaareguaranteedtofurthercultureintheconditionsprovidedbySciencellResearchLaboratories;however,MHMaarenotrecommendedforexpandingorlong-termculturessincethecellsdonotproliferateinregularculture.
RecommendedMedium
ItisrecommendedtouseMacrophageMedium(MaM,Cat.#1921)forculturingMHMainvitro.
Specifications:
CatalogNo. | M5340-57 |
CountryofManufacture | UnitedStates |
ProductCode | MHMa |
Size/Quantity | Eachvialcontains>1x10^6cellsin1mlvolume |
ProductUse | MHMaareforresearchuseonly.Theyarenotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
Storage | Uponreceiving,directlyandimmediatelytransferthecellsfromdryicetoliquidnitrogenandkeepthecellsinliquidnitrogenuntiltheyareneededforexperiments. |
ShippingInfo | Dryice. |
References | [1]LiaskouE,WilsonDV,OoYH.(2012)"Innateimmunecellsinliverinflammation."MediatorsInflamm.2012:949157.[2]BieghsV,VerheyenF,vanGorpPJ,HendrikxT,WoutersK,LutjohannD,GijbelsMJ,FebbraioM,BinderCJ,HofkerMH,Shiri-SverdlovR.(2012)"InternalizationofmodifiedlipidsbyCD36andSR-AleadstohepaticinflammationandlysosomalcholesterolstorageinKupffercells."PLoSOne.7:e34378.[3]TianY,JochumW,GeorgievP,MoritzW,GrafR,ClavienPA.(2006)"Kupffercell-dependentTNF-alphasignalingmediatesinjuryinthearterializedsmall-for-sizelivertransplantationinthemouse."ProcNatlAcadSciUSA.103:4598-603.[4]SekiE,deMinicisS,InokuchiS,TauraK,MiyaiK,vanRooijenN,SchwabeRF,BrennerDA.(2009)"CCR2promoteshepaticfibrosisinmice."Hepatology.50:185-97. |